Targeted RNA Kits

Targeted RNA-sequencing (RNA-Seq) is a highly accurate method for selecting and sequencing specific transcripts of interest and offers both quantitative and qualitative information. Targeted RNA-Seq can be achieved via either enrichment or amplicon-based approaches, both of which enable gene expression analysis in a focused set of genes of interest, with enrichment assays also providing the ability to detect both known and novel gene fusion partners in many sample types including formalin fixed paraffin embedded (FFPE) tissue.

RNA-enrichment provides quantitative expression information as well as the detection of small variants and gene fusions. RNA enrichment offers the following features:

• Compatible with difficult samples such as formalin fixed paraffin embedded (FFPE) tissue
• Low input requiring 10ng of total RNA or 20 – 100ng of FFPE RNA
• Detection of known and novel fusion gene partners
• Gene expression profiles with a broad dynamic range

RNA expression panels can be designed to focus on RNA sequences of interest or custom content can be added to fully optimized and experimentally validated panels.

• Offers a highly accurate and specific method for measuring transcripts of interest
• Provides qualitative and quantitative information for differential expression analysis, allele-specific expression measurement, and gene fusion verification
• Measures dozens to thousands of targets simultaneously
• Is compatible with low-quality or FFPE-derived RNA samples

TruSeq Targeted RNA Expression Kits
Fixed Panels:

• Apoptosis
• Cardiotoxicity
• Cell cycle
• Hedgehog pathway
• Neurodegeneration
• NFκB pathway
• p450 pathway
• p53 pathway
• Stem cells
• Wnt pathway

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